Protein Interaction Lab
About | What we are interested in...
Natural active compounds are used in modern medicine either directly isolated from plants or synthetically modified. Extracts of medicinal plants or natural products such as functional foods present an alternative to conventional drugs in the prevention and treatment of diseases. However, the underlying intrinsic biochemical mechanisms for these substances remain unclear in many cases. We are mainly interested in the interaction properties of membrane receptors and their binding partners. To detect these interactions we use an assay using micro-patterned surfaces. This µ-patterning assay provides a basis for the detection of the impact of secondary plant metabolites on important signal transduction molecules. In addition we focus on bioavailability studies and on the impact of secondary plant metabolites on RNA expression levels.
The findings will help to unravel the basic functions of these substances and provide a basis for the design of functional food.
Key technologies: Total internal reflection fluorescence microscopy (TIRFM) | Epi-fluorescence microscopy | Confocal microscopy | Fluorescence recovery after photobleaching (FRAP) | Micropatterning | Cell culture | HPLC | GC-MS | IC | Flow cytometry | Uptake studies (e.g. CaCo-2) | TEAC | ORAC | GLUT4-translocation | ICP | Protein-Phosphorylation assay | HET-CAM
Key facilities: Olympus IX81 TIRF/Epi-Fluorescence | Olympus IX2-DSU confocal unit | Olympus cellFRAP unit | Nikon Eclipse Ti2 | Andor FRAPPA | BMG POLARStar Platereader | Thermo UltiMate 3000 HPLC | Thermo ISQ QD singla quadrupole MS | Nucleofector device | Cell culture | Western Blot | Thermo GC-MS | Multichannel Ussingchamber | Horiba ICP-OES Ultima 2 | Dionex ICS-1000| Biorad C1000 thermal cycler and CFX96 real-time system | Jacso FT/IR 4100 spectrometer | Vilber Lourmat UV radiation system